diff --git a/scripts/pylib/twister/twisterlib/harness.py b/scripts/pylib/twister/twisterlib/harness.py
index f772c3410af..8e912716a25 100644
--- a/scripts/pylib/twister/twisterlib/harness.py
+++ b/scripts/pylib/twister/twisterlib/harness.py
@@ -389,7 +389,7 @@ class Pytest(Harness):
             f'--build-dir={self.running_dir}',
             f'--junit-xml={self.report_file}',
             '--log-file-level=DEBUG',
-            '--log-file-format=%(asctime)s.%(msecs)d:%(levelname)s:%(name)s: %(message)s',
+            '--log-file-format=%(asctime)s.%(msecs)03d:%(levelname)s:%(name)s: %(message)s',
             f'--log-file={self.pytest_log_file_path}',
             f'--platform={self.instance.platform.name}'
         ]