diff --git a/samples/bluetooth/unicast_audio_client/README.rst b/samples/bluetooth/unicast_audio_client/README.rst
index b9acf06d023..dc3b336d9a3 100644
--- a/samples/bluetooth/unicast_audio_client/README.rst
+++ b/samples/bluetooth/unicast_audio_client/README.rst
@@ -20,5 +20,7 @@ Building and Running
 ********************
 This sample can be found under
 :zephyr_file:`samples/bluetooth/unicast_audio_client` in the Zephyr tree.
+Use `-DEXTRA_CONF_FILE=overlay-bt_ll_sw_split.conf` to enable required ISO
+feature support in Zephyr Bluetooth Controller on supported boards.
 
 See :ref:`bluetooth samples section <bluetooth-samples>` for details.
diff --git a/samples/bluetooth/unicast_audio_client/overlay-bt_ll_sw_split.conf b/samples/bluetooth/unicast_audio_client/overlay-bt_ll_sw_split.conf
new file mode 100644
index 00000000000..cecb923bd35
--- /dev/null
+++ b/samples/bluetooth/unicast_audio_client/overlay-bt_ll_sw_split.conf
@@ -0,0 +1,8 @@
+CONFIG_BT_LL_SW_SPLIT=y
+CONFIG_BT_CTLR_SCAN_DATA_LEN_MAX=1650
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=4
+CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=155
+CONFIG_BT_CTLR_ISOAL_SOURCES=2
+CONFIG_BT_CTLR_ISOAL_SINKS=1
+CONFIG_BT_CTLR_ADVANCED_FEATURES=y
+CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
diff --git a/samples/bluetooth/unicast_audio_client/sample.yaml b/samples/bluetooth/unicast_audio_client/sample.yaml
index 1e9998c9770..9ca15182f52 100644
--- a/samples/bluetooth/unicast_audio_client/sample.yaml
+++ b/samples/bluetooth/unicast_audio_client/sample.yaml
@@ -10,3 +10,14 @@ tests:
     tags: bluetooth
     integration_platforms:
       - qemu_cortex_m3
+  sample.bluetooth.audio_unicast_client.bt_ll_sw_split:
+    harness: bluetooth
+    platform_allow:
+      - qemu_cortex_m3
+      - qemu_x86
+      - nrf52_bsim
+      - nrf52dk_nrf52832
+    integration_platforms:
+      - nrf52dk_nrf52832
+    extra_args: OVERLAY_CONFIG=overlay-bt_ll_sw_split.conf
+    tags: bluetooth
diff --git a/samples/bluetooth/unicast_audio_server/README.rst b/samples/bluetooth/unicast_audio_server/README.rst
index a94dc05f0ce..ce7151e5fb7 100644
--- a/samples/bluetooth/unicast_audio_server/README.rst
+++ b/samples/bluetooth/unicast_audio_server/README.rst
@@ -19,5 +19,7 @@ Building and Running
 ********************
 This sample can be found under
 :zephyr_file:`samples/bluetooth/unicast_audio_server` in the Zephyr tree.
+Use `-DEXTRA_CONF_FILE=overlay-bt_ll_sw_split.conf` to enable required ISO
+feature support in Zephyr Bluetooth Controller on supported boards.
 
 See :ref:`bluetooth samples section <bluetooth-samples>` for details.
diff --git a/samples/bluetooth/unicast_audio_server/overlay-bt_ll_sw_split.conf b/samples/bluetooth/unicast_audio_server/overlay-bt_ll_sw_split.conf
new file mode 100644
index 00000000000..a6b48df9c79
--- /dev/null
+++ b/samples/bluetooth/unicast_audio_server/overlay-bt_ll_sw_split.conf
@@ -0,0 +1,6 @@
+CONFIG_BT_LL_SW_SPLIT=y
+CONFIG_BT_CTLR_ADV_DATA_LEN_MAX=191
+CONFIG_BT_CTLR_ISO_TX_BUFFERS=2
+CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=155
+CONFIG_BT_CTLR_ISOAL_SOURCES=1
+CONFIG_BT_CTLR_ISOAL_SINKS=2
diff --git a/samples/bluetooth/unicast_audio_server/prj.conf b/samples/bluetooth/unicast_audio_server/prj.conf
index 37a0a5f4c6a..3aec74ddbcc 100644
--- a/samples/bluetooth/unicast_audio_server/prj.conf
+++ b/samples/bluetooth/unicast_audio_server/prj.conf
@@ -5,6 +5,7 @@ CONFIG_BT_AUDIO=y
 CONFIG_BT_BAP_UNICAST_SERVER=y
 CONFIG_BT_ASCS_ASE_SNK_COUNT=2
 CONFIG_BT_ASCS_ASE_SRC_COUNT=1
+CONFIG_BT_ISO_TX_BUF_COUNT=2
 # Support an ISO channel per ASE
 CONFIG_BT_ISO_MAX_CHAN=4
 
diff --git a/samples/bluetooth/unicast_audio_server/sample.yaml b/samples/bluetooth/unicast_audio_server/sample.yaml
index 477398a93f7..afb93362d1e 100644
--- a/samples/bluetooth/unicast_audio_server/sample.yaml
+++ b/samples/bluetooth/unicast_audio_server/sample.yaml
@@ -10,3 +10,14 @@ tests:
     tags: bluetooth
     integration_platforms:
       - qemu_cortex_m3
+  sample.bluetooth.audio_unicast_server.bt_ll_sw_split:
+    harness: bluetooth
+    platform_allow:
+      - qemu_cortex_m3
+      - qemu_x86
+      - nrf52_bsim
+      - nrf52dk_nrf52832
+    integration_platforms:
+      - nrf52dk_nrf52832
+    extra_args: OVERLAY_CONFIG=overlay-bt_ll_sw_split.conf
+    tags: bluetooth