diff --git a/tests/drivers/gpio/gpio_basic_api/boards/aw9523b_on_arduino_header.overlay b/tests/drivers/gpio/gpio_basic_api/boards/aw9523b_on_arduino_header.overlay
new file mode 100644
index 00000000000..5d086016c19
--- /dev/null
+++ b/tests/drivers/gpio/gpio_basic_api/boards/aw9523b_on_arduino_header.overlay
@@ -0,0 +1,25 @@
+&arduino_i2c {
+	status = "okay";
+	aw9523b@58 {
+		status = "okay";
+		reg = <0x58>;
+		compatible = "awinic,aw9523b";
+
+		aw9523_gpio: gpio {
+			compatible = "awinic,aw9523b-gpio";
+			gpio-controller;
+			#gpio-cells = <2>;
+			int-gpios = <&arduino_header 18 (GPIO_ACTIVE_LOW|GPIO_PULL_UP)>;
+			reset-gpios = <&arduino_header 19 (GPIO_ACTIVE_LOW|GPIO_PULL_UP)>;
+		};
+	};
+};
+
+/ {
+	resources {
+		compatible = "test-gpio-basic-api";
+		status = "okay";
+		out-gpios = <&aw9523_gpio 8 0>;
+		in-gpios = <&aw9523_gpio 9 0>;
+	};
+};
diff --git a/tests/drivers/gpio/gpio_basic_api/testcase.yaml b/tests/drivers/gpio/gpio_basic_api/testcase.yaml
index b2d23c34db8..052ec6be950 100644
--- a/tests/drivers/gpio/gpio_basic_api/testcase.yaml
+++ b/tests/drivers/gpio/gpio_basic_api/testcase.yaml
@@ -95,3 +95,14 @@ tests:
       - nucleo_wb05kz
       - nucleo_wb09ke
       - nucleo_wba55cg
+  drivers.gpio.2pin_aw9523b:
+    depends_on:
+      - gpio
+      - arduino_gpio
+      - i2c
+    harness_config:
+      fixture: aw9523b_on_arduino_header
+    filter: dt_compat_enabled("test-gpio-basic-api") and dt_compat_enabled("arduino-header-r3") and
+            dt_nodelabel_enabled("arduino_i2c")
+    extra_dtc_overlay_files:
+      - boards/aw9523b_on_arduino_header.overlay