diff --git a/.ruff-excludes.toml b/.ruff-excludes.toml
index e1d1b2c53b9..ced46706deb 100644
--- a/.ruff-excludes.toml
+++ b/.ruff-excludes.toml
@@ -795,7 +795,6 @@
     "SIM105",   # https://docs.astral.sh/ruff/rules/suppressible-exception
     "SIM114",   # https://docs.astral.sh/ruff/rules/if-with-same-arms
     "SIM115",   # https://docs.astral.sh/ruff/rules/open-file-with-context-handler
-    "SIM201",   # https://docs.astral.sh/ruff/rules/negate-equal-op
     "UP007",    # https://docs.astral.sh/ruff/rules/non-pep604-annotation
     "UP015",    # https://docs.astral.sh/ruff/rules/redundant-open-modes
     "UP030",    # https://docs.astral.sh/ruff/rules/format-literals
@@ -822,9 +821,6 @@
     "UP031",    # https://docs.astral.sh/ruff/rules/printf-string-formatting
     "UP032",    # https://docs.astral.sh/ruff/rules/f-string
 ]
-"./scripts/pylib/twister/twisterlib/jobserver.py" = [
-    "SIM201",   # https://docs.astral.sh/ruff/rules/negate-equal-op
-]
 "./scripts/pylib/twister/twisterlib/mixins.py" = [
     "UP004",    # https://docs.astral.sh/ruff/rules/useless-object-inheritance
 ]
@@ -856,7 +852,6 @@
     "F541",     # https://docs.astral.sh/ruff/rules/f-string-missing-placeholders
     "SIM102",   # https://docs.astral.sh/ruff/rules/collapsible-if
     "SIM115",   # https://docs.astral.sh/ruff/rules/open-file-with-context-handler
-    "SIM201",   # https://docs.astral.sh/ruff/rules/negate-equal-op
     "UP004",    # https://docs.astral.sh/ruff/rules/useless-object-inheritance
     "UP006",    # https://docs.astral.sh/ruff/rules/non-pep585-annotation
     "UP015",    # https://docs.astral.sh/ruff/rules/redundant-open-modes
@@ -890,7 +885,6 @@
     "F541",     # https://docs.astral.sh/ruff/rules/f-string-missing-placeholders
     "SIM102",   # https://docs.astral.sh/ruff/rules/collapsible-if
     "SIM110",   # https://docs.astral.sh/ruff/rules/reimplemented-builtin
-    "SIM202",   # https://docs.astral.sh/ruff/rules/negate-not-equal-op
     "UP015",    # https://docs.astral.sh/ruff/rules/redundant-open-modes
     "UP031",    # https://docs.astral.sh/ruff/rules/printf-string-formatting
     "UP032",    # https://docs.astral.sh/ruff/rules/f-string
diff --git a/scripts/pylib/twister/twisterlib/handlers.py b/scripts/pylib/twister/twisterlib/handlers.py
index 72e2eaf0067..5322e1d1ecf 100755
--- a/scripts/pylib/twister/twisterlib/handlers.py
+++ b/scripts/pylib/twister/twisterlib/handlers.py
@@ -116,7 +116,7 @@ class Handler:
         logger.debug(f"Expected suite names:{expected_suite_names}")
         logger.debug(f"Detected suite names:{detected_suite_names}")
         if not expected_suite_names or \
-                not harness_status == TwisterStatus.PASS:
+                harness_status != TwisterStatus.PASS:
             return
         if not detected_suite_names:
             self._missing_suite_name(expected_suite_names, handler_time)
diff --git a/scripts/pylib/twister/twisterlib/jobserver.py b/scripts/pylib/twister/twisterlib/jobserver.py
index 731f9fc3556..d004a451c14 100644
--- a/scripts/pylib/twister/twisterlib/jobserver.py
+++ b/scripts/pylib/twister/twisterlib/jobserver.py
@@ -150,14 +150,14 @@ class GNUMakeJobClient(JobClient):
                     # Use F_GETFL to see if file descriptors are valid
                     if pipe:
                         rc = fcntl.fcntl(pipe[0], fcntl.F_GETFL)
-                        if not rc & os.O_ACCMODE == os.O_RDONLY:
+                        if rc & os.O_ACCMODE != os.O_RDONLY:
                             logger.warning(
                                 "FD %s is not readable (flags=%x); "
                                 "ignoring GNU make jobserver", pipe[0], rc)
                             pipe = None
                     if pipe:
                         rc = fcntl.fcntl(pipe[1], fcntl.F_GETFL)
-                        if not rc & os.O_ACCMODE == os.O_WRONLY:
+                        if rc & os.O_ACCMODE != os.O_WRONLY:
                             logger.warning(
                                 "FD %s is not writable (flags=%x); "
                                 "ignoring GNU make jobserver", pipe[1], rc)
diff --git a/scripts/pylib/twister/twisterlib/runner.py b/scripts/pylib/twister/twisterlib/runner.py
index e7ad7987d77..04200fef362 100644
--- a/scripts/pylib/twister/twisterlib/runner.py
+++ b/scripts/pylib/twister/twisterlib/runner.py
@@ -1730,7 +1730,7 @@ class TwisterRunner:
         the static filter stats. So need to prepare them before pipline starts.
         '''
         for instance in self.instances.values():
-            if instance.status == TwisterStatus.FILTER and not instance.reason == 'runtime filter':
+            if instance.status == TwisterStatus.FILTER and instance.reason != 'runtime filter':
                 self.results.filtered_static_increment()
                 self.results.filtered_configs_increment()
                 self.results.filtered_cases_increment(len(instance.testsuite.testcases))