diff --git a/subsys/bluetooth/controller/ll_sw/ull_peripheral_iso.c b/subsys/bluetooth/controller/ll_sw/ull_peripheral_iso.c
index d45d89aa009..f68ca66656f 100644
--- a/subsys/bluetooth/controller/ll_sw/ull_peripheral_iso.c
+++ b/subsys/bluetooth/controller/ll_sw/ull_peripheral_iso.c
@@ -250,7 +250,8 @@ uint8_t ull_peripheral_iso_acquire(struct ll_conn *acl,
 	cis->p_max_sdu = (uint16_t)(req->p_max_sdu[1] & 0x0F) << 8 |
 				    req->p_max_sdu[0];
 
-	cis->lll.handle = 0xFFFF;
+	cis->lll.active = 0U;
+	cis->lll.handle = LLL_HANDLE_INVALID;
 	cis->lll.acl_handle = acl->lll.handle;
 	cis->lll.sub_interval = sys_get_le24(req->sub_interval);
 	cis->lll.nse = req->nse;
@@ -325,7 +326,6 @@ uint8_t ull_peripheral_iso_setup(struct pdu_data_llctrl_cis_ind *ind,
 	cis->lll.cie = 0U;
 	cis->lll.npi = 0U;
 	cis->lll.flush = LLL_CIS_FLUSH_NONE;
-	cis->lll.active = 0U;
 	cis->lll.datapath_ready_rx = 0U;
 	cis->lll.tx.payload_count = 0U;
 	cis->lll.rx.payload_count = 0U;