diff --git a/boards/adi/max32662evkit/max32662evkit.dts b/boards/adi/max32662evkit/max32662evkit.dts
index ed1d597eb0d..e458a8d6904 100644
--- a/boards/adi/max32662evkit/max32662evkit.dts
+++ b/boards/adi/max32662evkit/max32662evkit.dts
@@ -70,6 +70,12 @@
 	status = "okay";
 };
 
+&i2c1 {
+	status = "okay";
+	pinctrl-0 = <&i2c1a_scl_p0_6 &i2c1a_sda_p0_9>;
+	pinctrl-names = "default";
+};
+
 &trng {
 	status = "okay";
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/max32662evkit.conf b/tests/drivers/i2c/i2c_target_api/boards/max32662evkit.conf
new file mode 100644
index 00000000000..c25d2c5d47f
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/max32662evkit.conf
@@ -0,0 +1,4 @@
+# Copyright (c) 2024 Analog Devices, Inc.
+# SPDX-License-Identifier: Apache-2.0
+
+CONFIG_I2C_VIRTUAL=n
diff --git a/tests/drivers/i2c/i2c_target_api/boards/max32662evkit.overlay b/tests/drivers/i2c/i2c_target_api/boards/max32662evkit.overlay
new file mode 100644
index 00000000000..1f78e3a1be6
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/max32662evkit.overlay
@@ -0,0 +1,28 @@
+/*
+ * Copyright (c) 2024 Analog Devices, Inc.
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+&i2c0 {
+	status = "okay";
+	pinctrl-0 = <&i2c0a_scl_p0_12 &i2c0a_sda_p0_13>;
+	pinctrl-names = "default";
+
+	eeprom0: eeprom@54 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x54>;
+		size = <256>;
+	};
+};
+
+&i2c1 {
+	dmas = <&dma0 1 MAX32_DMA_SLOT_I2C1_TX>, <&dma0 2 MAX32_DMA_SLOT_I2C1_RX>;
+	dma-names = "tx", "rx";
+
+	eeprom1: eeprom@56 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x56>;
+		size = <256>;
+	};
+};