diff --git a/tests/drivers/spi/spi_loopback/overlay-stm32-spi-dma.conf b/tests/drivers/spi/spi_loopback/overlay-stm32-spi-dma.conf
new file mode 100644
index 00000000000..3ce3d8ee097
--- /dev/null
+++ b/tests/drivers/spi/spi_loopback/overlay-stm32-spi-dma.conf
@@ -0,0 +1,5 @@
+# enable DMA mode for SPI loopback test
+CONFIG_SPI_LOOPBACK_DRV_NAME="SPI_1"
+CONFIG_SPI_STM32_DMA=y
+CONFIG_SPI_STM32_INTERRUPT=n
+CONFIG_SPI_ASYNC=n
diff --git a/tests/drivers/spi/spi_loopback/overlay-stm32-spi.conf b/tests/drivers/spi/spi_loopback/overlay-stm32-spi.conf
new file mode 100644
index 00000000000..733137888e3
--- /dev/null
+++ b/tests/drivers/spi/spi_loopback/overlay-stm32-spi.conf
@@ -0,0 +1,2 @@
+# enable stm32 spi instance for loopback test
+CONFIG_SPI_LOOPBACK_DRV_NAME="SPI_1"
diff --git a/tests/drivers/spi/spi_loopback/testcase.yaml b/tests/drivers/spi/spi_loopback/testcase.yaml
index 08841e4637e..6653d80acca 100644
--- a/tests/drivers/spi/spi_loopback/testcase.yaml
+++ b/tests/drivers/spi/spi_loopback/testcase.yaml
@@ -6,6 +6,7 @@ common:
 tests:
   drivers.spi.loopback:
     harness: ztest
+    filter: not CONFIG_SOC_FAMILY_STM32
     harness_config:
       fixture: spi_loopback
   driver.spi.loopback.internal:
@@ -17,3 +18,18 @@ tests:
     harness_config:
       fixture: spi_loopback
     platform_allow: frdm_k64f
+  drivers.stm32_spi.loopback:
+    harness: ztest
+    extra_args: OVERLAY_CONFIG="overlay-stm32-spi.conf"
+    harness_config:
+      fixture: spi_loopback
+    filter: CONFIG_SOC_FAMILY_STM32
+  drivers.stm32_spi_dma.loopback:
+    tags: dma
+    harness: ztest
+    harness_config:
+      fixture: spi_loopback
+    extra_args: OVERLAY_CONFIG="overlay-stm32-spi-dma.conf"
+    filter: CONFIG_SOC_FAMILY_STM32
+    platform_allow: nucleo_g474re nucleo_f207zg nucleo_f429zi nucleo_f746zg nucleo_wb55rg
+        nucleo_wl55jc nucleo_h743zi