diff --git a/tests/drivers/i2c/i2c_target_api/boards/rpi_pico.overlay b/tests/drivers/i2c/i2c_target_api/boards/rpi_pico.overlay
index 2b8d6b7382b..12524053fa7 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/rpi_pico.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/rpi_pico.overlay
@@ -4,6 +4,16 @@
  * SPDX-License-Identifier: Apache-2.0
  */
 
+&pinctrl {
+	i2c1_default: i2c1_default {
+		group1 {
+			pinmux = <I2C1_SDA_P14>, <I2C1_SCL_P15>;
+			input-enable;
+			input-schmitt-enable;
+		};
+	};
+};
+
 &i2c0 {
 	eeprom0: eeprom@54 {
 		compatible = "atmel,at24";
@@ -16,6 +26,10 @@
 };
 
 &i2c1 {
+	clock-frequency = <I2C_BITRATE_STANDARD>;
+	status = "okay";
+	pinctrl-0 = <&i2c1_default>;
+	pinctrl-names = "default";
 	eeprom1: eeprom@56 {
 		compatible = "atmel,at24";
 		reg = <0x56>;