diff --git a/boards/st/nucleo_u5a5zj_q/doc/index.rst b/boards/st/nucleo_u5a5zj_q/doc/index.rst
index 60701060fcc..ea919c4f10e 100644
--- a/boards/st/nucleo_u5a5zj_q/doc/index.rst
+++ b/boards/st/nucleo_u5a5zj_q/doc/index.rst
@@ -236,7 +236,7 @@ Default Zephyr Peripheral Mapping:
 - LD3 : PG2
 - LPUART_1_TX : PG7
 - LPUART_1_RX : PG8
-- SPI_1_NSS : PA4
+- SPI_1 nCS (GPIO) : PD14
 - SPI_1_SCK : PA5
 - SPI_1_MISO : PA6
 - SPI_1_MOSI : PA7
diff --git a/boards/st/nucleo_u5a5zj_q/nucleo_u5a5zj_q-common.dtsi b/boards/st/nucleo_u5a5zj_q/nucleo_u5a5zj_q-common.dtsi
index a08d7b47ce2..ecc9e862c99 100644
--- a/boards/st/nucleo_u5a5zj_q/nucleo_u5a5zj_q-common.dtsi
+++ b/boards/st/nucleo_u5a5zj_q/nucleo_u5a5zj_q-common.dtsi
@@ -112,9 +112,9 @@
 };
 
 &spi1 {
-	pinctrl-0 = <&spi1_nss_pe12 &spi1_sck_pa5
-		     &spi1_miso_pa6 &spi1_mosi_pa7>;
+	pinctrl-0 = <&spi1_sck_pa5 &spi1_miso_pa6 &spi1_mosi_pa7>;
 	pinctrl-names = "default";
+	cs-gpios = <&gpiod 14 GPIO_ACTIVE_LOW>;
 	status = "okay";
 };