diff --git a/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340dk_nrf5340_cpuapp.conf b/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340dk_nrf5340_cpuapp.conf
new file mode 100644
index 00000000000..b77788bd0e5
--- /dev/null
+++ b/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340dk_nrf5340_cpuapp.conf
@@ -0,0 +1,2 @@
+CONFIG_COUNTER=y
+CONFIG_COUNTER_TIMER0=y
diff --git a/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340dk_nrf5340_cpuapp.overlay b/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340dk_nrf5340_cpuapp.overlay
new file mode 100644
index 00000000000..277ee171f0d
--- /dev/null
+++ b/tests/drivers/uart/uart_mix_fifo_poll/boards/nrf5340dk_nrf5340_cpuapp.overlay
@@ -0,0 +1,36 @@
+/* SPDX-License-Identifier: Apache-2.0 */
+
+&pinctrl {
+	uart1_default_alt: uart1_default_alt {
+		group1 {
+			psels = <NRF_PSEL(UART_TX, 0, 4)>,
+				<NRF_PSEL(UART_RX, 0, 5)>,
+				<NRF_PSEL(UART_RTS, 0, 6)>,
+				<NRF_PSEL(UART_CTS, 0, 7)>;
+		};
+	};
+
+	uart1_sleep_alt: uart1_sleep_alt {
+		group1 {
+			psels = <NRF_PSEL(UART_TX, 0, 4)>,
+				<NRF_PSEL(UART_RX, 0, 5)>,
+				<NRF_PSEL(UART_RTS, 0, 6)>,
+				<NRF_PSEL(UART_CTS, 0, 7)>;
+			low-power-enable;
+		};
+	};
+};
+
+&uart1 {
+	current-speed = <115200>;
+	compatible = "nordic,nrf-uarte";
+	status = "okay";
+	pinctrl-0 = <&uart1_default_alt>;
+	pinctrl-1 = <&uart1_sleep_alt>;
+	pinctrl-names = "default", "sleep";
+	hw-flow-control;
+};
+
+&rtc1 {
+	interrupts = <21 2>;
+};
diff --git a/tests/drivers/uart/uart_mix_fifo_poll/src/main.c b/tests/drivers/uart/uart_mix_fifo_poll/src/main.c
index 41c320f4acc..bc67d4327b9 100644
--- a/tests/drivers/uart/uart_mix_fifo_poll/src/main.c
+++ b/tests/drivers/uart/uart_mix_fifo_poll/src/main.c
@@ -19,6 +19,8 @@
 
 #if defined(CONFIG_BOARD_NRF52840DK_NRF52840)
 #define UART_DEVICE_DEV DT_NODELABEL(uart0)
+#elif defined(CONFIG_BOARD_NRF5340DK_NRF5340_CPUAPP)
+#define UART_DEVICE_DEV DT_NODELABEL(uart1)
 #elif defined(CONFIG_BOARD_NRF9160DK_NRF9160)
 #define UART_DEVICE_DEV DT_NODELABEL(uart1)
 #elif defined(CONFIG_BOARD_ATSAMD21_XPRO)
diff --git a/tests/drivers/uart/uart_mix_fifo_poll/testcase.yaml b/tests/drivers/uart/uart_mix_fifo_poll/testcase.yaml
index b16aeeeefe6..caf9c024299 100644
--- a/tests/drivers/uart/uart_mix_fifo_poll/testcase.yaml
+++ b/tests/drivers/uart/uart_mix_fifo_poll/testcase.yaml
@@ -1,7 +1,7 @@
 common:
   tags: drivers uart
   harness: ztest
-  platform_allow: nrf52840dk_nrf52840 nrf9160dk_nrf9160
+  platform_allow: nrf52840dk_nrf52840 nrf9160dk_nrf9160 nrf5340dk_nrf5340_cpuapp
   integration_platforms:
     - nrf52840dk_nrf52840
   harness_config: