diff --git a/scripts/pylib/twister/twisterlib/runner.py b/scripts/pylib/twister/twisterlib/runner.py
index 9e4cf2443ac..f5e2cabf74d 100644
--- a/scripts/pylib/twister/twisterlib/runner.py
+++ b/scripts/pylib/twister/twisterlib/runner.py
@@ -20,6 +20,7 @@ from multiprocessing import Lock, Process, Value
 from multiprocessing.managers import BaseManager
 from typing import List
 from packaging import version
+import pathlib
 
 from colorama import Fore
 from domains import Domains
@@ -363,7 +364,8 @@ class CMake:
             f'-DTC_NAME={self.instance.testsuite.name}',
             f'-D{warning_command}={warnings_as_errors}',
             f'-DEXTRA_GEN_EDT_ARGS={gen_edt_args}',
-            f'-G{self.env.generator}'
+            f'-G{self.env.generator}',
+            f'-DPython3_EXECUTABLE={pathlib.Path(sys.executable).as_posix()}'
         ]
 
         # If needed, run CMake using the package_helper script first, to only run
diff --git a/scripts/tests/twister/test_runner.py b/scripts/tests/twister/test_runner.py
index e0a0ca51194..d2d0143d189 100644
--- a/scripts/tests/twister/test_runner.py
+++ b/scripts/tests/twister/test_runner.py
@@ -373,6 +373,7 @@ TESTDATA_2_2 = [
       '-B' + os.path.join('build', 'dir'), '-DTC_RUNID=1', '-DTC_NAME=testcase',
       '-DSB_CONFIG_COMPILER_WARNINGS_AS_ERRORS=y',
       '-DEXTRA_GEN_EDT_ARGS=--edtlib-Werror', '-Gdummy_generator',
+      f'-DPython3_EXECUTABLE={pathlib.Path(sys.executable).as_posix()}',
       '-S' + os.path.join('source', 'dir'),
       'arg1', 'arg2',
       '-DBOARD=<platform name>',
@@ -387,6 +388,7 @@ TESTDATA_2_2 = [
       '-B' + os.path.join('build', 'dir'), '-DTC_RUNID=1', '-DTC_NAME=testcase',
       '-DSB_CONFIG_COMPILER_WARNINGS_AS_ERRORS=n',
       '-DEXTRA_GEN_EDT_ARGS=', '-Gdummy_generator',
+      f'-DPython3_EXECUTABLE={pathlib.Path(sys.executable).as_posix()}',
       '-Szephyr_base/share/sysbuild',
       '-DAPP_DIR=' + os.path.join('source', 'dir'),
       'arg1', 'arg2',