diff --git a/tests/drivers/adc/adc_api/boards/nucleo_f091rc.overlay b/tests/drivers/adc/adc_api/boards/nucleo_f091rc.overlay
index f0d65c1e941..d8d89d460a0 100644
--- a/tests/drivers/adc/adc_api/boards/nucleo_f091rc.overlay
+++ b/tests/drivers/adc/adc_api/boards/nucleo_f091rc.overlay
@@ -7,11 +7,12 @@
 / {
 	zephyr,user {
 		/* adjust channel number according to pinmux in board.dts */
-		io-channels = <&adc1 0>;
+		io-channels = <&adc1 0>, <&adc1 1>;
 	};
 };
 
 &adc1 {
+	pinctrl-0 = <&adc_in0_pa0 &adc_in1_pa1>;
 	#address-cells = <1>;
 	#size-cells = <0>;
 
@@ -19,7 +20,15 @@
 		reg = <0>;
 		zephyr,gain = "ADC_GAIN_1";
 		zephyr,reference = "ADC_REF_INTERNAL";
-		zephyr,acquisition-time = <ADC_ACQ_TIME_DEFAULT>;
+		zephyr,acquisition-time = <ADC_ACQ_TIME_MAX>;
+		zephyr,resolution = <12>;
+	};
+
+	channel@1 {
+		reg = <1>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <ADC_ACQ_TIME_MAX>;
 		zephyr,resolution = <12>;
 	};
 };