diff --git a/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm33.conf b/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm33.conf
new file mode 100644
index 00000000000..70e009fae68
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm33.conf
@@ -0,0 +1 @@
+CONFIG_I2C_VIRTUAL=n
diff --git a/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm33.overlay b/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm33.overlay
new file mode 100644
index 00000000000..f521284519c
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm33.overlay
@@ -0,0 +1,25 @@
+/*
+ * Copyright 2024 NXP
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/* To test this sample, connect J41.12 <-> J44.20 and J41.10 <-> J44.18 */
+
+&lpi2c2 {
+	status = "okay";
+	eeprom0: eeprom@54 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x54>;
+		size = <256>;
+	};
+};
+
+&lpi2c3 {
+	status = "okay";
+	eeprom1: eeprom@56 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x56>;
+		size = <256>;
+	};
+};
diff --git a/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm7.conf b/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm7.conf
new file mode 100644
index 00000000000..70e009fae68
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm7.conf
@@ -0,0 +1 @@
+CONFIG_I2C_VIRTUAL=n
diff --git a/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm7.overlay b/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm7.overlay
new file mode 100644
index 00000000000..f521284519c
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/mimxrt1180_evk_mimxrt1189_cm7.overlay
@@ -0,0 +1,25 @@
+/*
+ * Copyright 2024 NXP
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/* To test this sample, connect J41.12 <-> J44.20 and J41.10 <-> J44.18 */
+
+&lpi2c2 {
+	status = "okay";
+	eeprom0: eeprom@54 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x54>;
+		size = <256>;
+	};
+};
+
+&lpi2c3 {
+	status = "okay";
+	eeprom1: eeprom@56 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x56>;
+		size = <256>;
+	};
+};
diff --git a/tests/drivers/i2c/i2c_target_api/testcase.yaml b/tests/drivers/i2c/i2c_target_api/testcase.yaml
index 0087a00ba8e..ec36886b1ca 100644
--- a/tests/drivers/i2c/i2c_target_api/testcase.yaml
+++ b/tests/drivers/i2c/i2c_target_api/testcase.yaml
@@ -37,6 +37,8 @@ tests:
       - frdm_mcxn947/mcxn947/cpu0
       - mimxrt1170_evk@B/mimxrt1176/cm7
       - mimxrt1170_evk/mimxrt1176/cm7
+      - mimxrt1180_evk/mimxrt1189/cm33
+      - mimxrt1180_evk/mimxrt1189/cm7
       - mimxrt1040_evk
       - mimxrt1060_evk
       - frdm_ke17z512