diff --git a/boards/arm/nrf5340dk_nrf5340/nrf5340_cpuapp_common.dts b/boards/arm/nrf5340dk_nrf5340/nrf5340_cpuapp_common.dts
index c2636623ffe..99d1bfa25ff 100644
--- a/boards/arm/nrf5340dk_nrf5340/nrf5340_cpuapp_common.dts
+++ b/boards/arm/nrf5340dk_nrf5340/nrf5340_cpuapp_common.dts
@@ -104,14 +104,6 @@
 	ch0-pin = <28>;
 };
 
-&spi2 {
-	compatible = "nordic,nrf-spim";
-	status = "okay";
-	sck-pin = <47>;
-	miso-pin = <46>;
-	mosi-pin = <45>;
-};
-
 &timer0 {
 	status = "okay";
 };
diff --git a/boards/arm/nrf5340dk_nrf5340/nrf5340dk_nrf5340_cpuapp.dts b/boards/arm/nrf5340dk_nrf5340/nrf5340dk_nrf5340_cpuapp.dts
index da1091b03c4..45fb1ca5689 100644
--- a/boards/arm/nrf5340dk_nrf5340/nrf5340dk_nrf5340_cpuapp.dts
+++ b/boards/arm/nrf5340dk_nrf5340/nrf5340dk_nrf5340_cpuapp.dts
@@ -20,3 +20,11 @@
 		zephyr,sram-non-secure-partition = &sram0_ns;
 	};
 };
+
+&spi2 {
+	compatible = "nordic,nrf-spim";
+	status = "okay";
+	sck-pin = <47>;
+	miso-pin = <46>;
+	mosi-pin = <45>;
+};
diff --git a/dts/arm/nordic/nrf5340_cpuapp_qkaa_eng_a.dtsi b/dts/arm/nordic/nrf5340_cpuapp_qkaa_eng_a.dtsi
index 12f76c89b86..1142990bfa5 100644
--- a/dts/arm/nordic/nrf5340_cpuapp_qkaa_eng_a.dtsi
+++ b/dts/arm/nordic/nrf5340_cpuapp_qkaa_eng_a.dtsi
@@ -24,3 +24,16 @@
 		compatible = "nordic,nRF5340-CPUAPP-QKAA", "nordic,nRF5340-CPUAPP", "nordic,nRF53", "simple-bus";
 	};
 };
+
+/*
+ * Several peripherals are not functional on ENG_A revision
+ * so we delete the corresponding DTS nodes.
+ */
+/delete-node/ &i2c2;
+/delete-node/ &i2c3;
+/delete-node/ &spi2;
+/delete-node/ &spi3;
+/delete-node/ &uart2;
+/delete-node/ &uart3;
+/delete-node/ &pwm3;
+/delete-node/ &wdt1;