diff --git a/samples/bluetooth/broadcast_audio_sink/README.rst b/samples/bluetooth/broadcast_audio_sink/README.rst
index 68aeffd7413..f733dc201cf 100644
--- a/samples/bluetooth/broadcast_audio_sink/README.rst
+++ b/samples/bluetooth/broadcast_audio_sink/README.rst
@@ -20,5 +20,7 @@ Building and Running
 ********************
 This sample can be found under
 :zephyr_file:`samples/bluetooth/broadcast_audio_sink` in the Zephyr tree.
+Use `-DOVERLAY_CONFIG=overlay-bt_ll_sw_split.conf` to enable required ISO
+feature support in Zephyr Bluetooth Controller on supported boards.
 
 See :ref:`bluetooth samples section <bluetooth-samples>` for details.
diff --git a/samples/bluetooth/broadcast_audio_sink/boards/nrf52840dk_nrf52840.conf b/samples/bluetooth/broadcast_audio_sink/boards/nrf52840dk_nrf52840.conf
deleted file mode 100644
index 540990105f3..00000000000
--- a/samples/bluetooth/broadcast_audio_sink/boards/nrf52840dk_nrf52840.conf
+++ /dev/null
@@ -1,6 +0,0 @@
-CONFIG_BT_CTLR_SYNC_ISO=y
-
-# Supports the highest SDU size required by any BAP LC3 presets (155)
-CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=155
-# Supports the highest advertising data that can be set in a single HCI command
-CONFIG_BT_CTLR_SCAN_DATA_LEN_MAX=251
diff --git a/samples/bluetooth/broadcast_audio_sink/boards/nrf52_bsim.conf b/samples/bluetooth/broadcast_audio_sink/boards/nrf52_bsim.conf
deleted file mode 100644
index 540990105f3..00000000000
--- a/samples/bluetooth/broadcast_audio_sink/boards/nrf52_bsim.conf
+++ /dev/null
@@ -1,6 +0,0 @@
-CONFIG_BT_CTLR_SYNC_ISO=y
-
-# Supports the highest SDU size required by any BAP LC3 presets (155)
-CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=155
-# Supports the highest advertising data that can be set in a single HCI command
-CONFIG_BT_CTLR_SCAN_DATA_LEN_MAX=251
diff --git a/samples/bluetooth/broadcast_audio_sink/overlay-bt_ll_sw_split.conf b/samples/bluetooth/broadcast_audio_sink/overlay-bt_ll_sw_split.conf
new file mode 100644
index 00000000000..bc0ba2c6cdd
--- /dev/null
+++ b/samples/bluetooth/broadcast_audio_sink/overlay-bt_ll_sw_split.conf
@@ -0,0 +1,12 @@
+# Zephyr Bluetooth Controller
+CONFIG_BT_LL_SW_SPLIT=y
+
+# Enable support for Broadcast ISO Sync
+CONFIG_BT_CTLR_SYNC_ISO=y
+
+# Supports the highest SDU size required by any BAP LC3 presets (155)
+CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=155
+
+# Supports the highest advertising data that is set in a single HCI command in
+# Zephyr Bluetooth Controller
+CONFIG_BT_CTLR_SCAN_DATA_LEN_MAX=191
diff --git a/samples/bluetooth/broadcast_audio_sink/sample.yaml b/samples/bluetooth/broadcast_audio_sink/sample.yaml
index f184f1610e4..48318561bf0 100644
--- a/samples/bluetooth/broadcast_audio_sink/sample.yaml
+++ b/samples/bluetooth/broadcast_audio_sink/sample.yaml
@@ -4,7 +4,21 @@ sample:
 tests:
   sample.bluetooth.broadcast_audio_sink:
     harness: bluetooth
-    platform_allow: qemu_cortex_m3 qemu_x86 nrf52_bsim
-    tags: bluetooth
+    platform_allow: >
+      qemu_cortex_m3
+      qemu_x86
+      nrf5340dk_nrf5340_cpuapp
     integration_platforms:
-      - qemu_cortex_m3
+      - qemu_x86
+      - nrf5340dk_nrf5340_cpuapp
+    tags: bluetooth
+  sample.bluetooth.broadcast_audio_sink.bt_ll_sw_split:
+    harness: bluetooth
+    platform_allow: >
+      nrf52833dk_nrf52820
+      nrf52833dk_nrf52833
+    integration_platforms:
+      - nrf52_bsim
+      - nrf52833dk_nrf52833
+    extra_args: OVERLAY_CONFIG=overlay-bt_ll_sw_split.conf
+    tags: bluetooth
diff --git a/samples/bluetooth/broadcast_audio_source/README.rst b/samples/bluetooth/broadcast_audio_source/README.rst
index 00a90d0de04..223fa27443d 100644
--- a/samples/bluetooth/broadcast_audio_source/README.rst
+++ b/samples/bluetooth/broadcast_audio_source/README.rst
@@ -23,5 +23,7 @@ Building and Running
 ********************
 This sample can be found under
 :zephyr_file:`samples/bluetooth/broadcast_audio_source` in the Zephyr tree.
+Use `-DOVERLAY_CONFIG=overlay-bt_ll_sw_split.conf` to enable required ISO
+feature support in Zephyr Bluetooth Controller on supported boards.
 
 See :ref:`bluetooth samples section <bluetooth-samples>` for details.
diff --git a/samples/bluetooth/broadcast_audio_source/boards/nrf52840dk_nrf52840.conf b/samples/bluetooth/broadcast_audio_source/boards/nrf52840dk_nrf52840.conf
deleted file mode 100644
index c5879d03d8b..00000000000
--- a/samples/bluetooth/broadcast_audio_source/boards/nrf52840dk_nrf52840.conf
+++ /dev/null
@@ -1,7 +0,0 @@
-CONFIG_BT_CTLR_ADV_ISO=y
-# Supports the highest SDU size required by any BAP LC3 presets (155)
-CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=155
-# Sufficient ISO PDU length for any BAP LC3 presets (155)
-CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=155
-# Zephyr Controller tested maximum advertising data that can be set in a single HCI command
-CONFIG_BT_CTLR_ADV_DATA_LEN_MAX=191
diff --git a/samples/bluetooth/broadcast_audio_source/boards/nrf52_bsim.conf b/samples/bluetooth/broadcast_audio_source/boards/nrf52_bsim.conf
deleted file mode 100644
index c5879d03d8b..00000000000
--- a/samples/bluetooth/broadcast_audio_source/boards/nrf52_bsim.conf
+++ /dev/null
@@ -1,7 +0,0 @@
-CONFIG_BT_CTLR_ADV_ISO=y
-# Supports the highest SDU size required by any BAP LC3 presets (155)
-CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=155
-# Sufficient ISO PDU length for any BAP LC3 presets (155)
-CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=155
-# Zephyr Controller tested maximum advertising data that can be set in a single HCI command
-CONFIG_BT_CTLR_ADV_DATA_LEN_MAX=191
diff --git a/samples/bluetooth/broadcast_audio_source/boards/nrf5340dk_nrf5340_cpuapp.conf b/samples/bluetooth/broadcast_audio_source/boards/nrf5340dk_nrf5340_cpuapp.conf
deleted file mode 100644
index fbf93ce3ea9..00000000000
--- a/samples/bluetooth/broadcast_audio_source/boards/nrf5340dk_nrf5340_cpuapp.conf
+++ /dev/null
@@ -1 +0,0 @@
-CONFIG_BT_CTLR_ADV_ISO=y
diff --git a/samples/bluetooth/broadcast_audio_source/overlay-bt_ll_sw_split.conf b/samples/bluetooth/broadcast_audio_source/overlay-bt_ll_sw_split.conf
new file mode 100644
index 00000000000..c73ff9c3ea9
--- /dev/null
+++ b/samples/bluetooth/broadcast_audio_source/overlay-bt_ll_sw_split.conf
@@ -0,0 +1,24 @@
+# Zephyr Bluetooth Controller
+CONFIG_BT_LL_SW_SPLIT=y
+
+# Zephyr Controller tested maximum advertising data that can be set in a single HCI command
+CONFIG_BT_CTLR_ADV_DATA_LEN_MAX=191
+
+# Enable support for Broadcast ISO in Zephyr Bluetooth Controller
+CONFIG_BT_CTLR_ADV_ISO=y
+
+# Sufficient ISO PDU length for any BAP LC3 presets (155)
+CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=155
+
+# Number of supported streams
+CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_ISOAL_SOURCES=2
+
+# FIXME: Host needs CONFIG_BT_ISO_TX_MTU + 4 bytes for sequence number, and  optionally
+# additional + 4 bytes for timestamp when not using BT_ISO_TIMESTAMP_NONE in bt_iso_chan_send(),
+# otherwise Host tries to fragment ISO data.
+# When Host is fixed, CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE can inherit the
+# CONFIG_BT_ISO_TX_MTU value.
+#
+# Supports the highest SDU size required by any BAP LC3 presets (155)
+CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=163
diff --git a/samples/bluetooth/broadcast_audio_source/sample.yaml b/samples/bluetooth/broadcast_audio_source/sample.yaml
index b48e1f67d21..c3a59940eb5 100644
--- a/samples/bluetooth/broadcast_audio_source/sample.yaml
+++ b/samples/bluetooth/broadcast_audio_source/sample.yaml
@@ -4,7 +4,21 @@ sample:
 tests:
   sample.bluetooth.broadcast_audio_source:
     harness: bluetooth
-    platform_allow: qemu_cortex_m3 qemu_x86 nrf52_bsim
+    platform_allow: >
+      qemu_cortex_m3
+      qemu_x86
+      nrf5340dk_nrf5340_cpuapp
     integration_platforms:
       - qemu_x86
+      - nrf5340dk_nrf5340_cpuapp
+    tags: bluetooth
+  sample.bluetooth.broadcast_audio_sink.bt_ll_sw_split:
+    harness: bluetooth
+    platform_allow: >
+      nrf52833dk_nrf52820
+      nrf52833dk_nrf52833
+    integration_platforms:
+      - nrf52_bsim
+      - nrf52833dk_nrf52833
+    extra_args: OVERLAY_CONFIG=overlay-bt_ll_sw_split.conf
     tags: bluetooth