diff --git a/tests/drivers/i2c/i2c_target_api/boards/b_u585i_iot02a.overlay b/tests/drivers/i2c/i2c_target_api/boards/b_u585i_iot02a.overlay
index c1d15d027cc..9940982aefb 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/b_u585i_iot02a.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/b_u585i_iot02a.overlay
@@ -16,7 +16,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -24,6 +24,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/efr32bg22_brd4184a.overlay b/tests/drivers/i2c/i2c_target_api/boards/efr32bg22_brd4184a.overlay
index ba0a0ce28f0..a68e23a44cb 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/efr32bg22_brd4184a.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/efr32bg22_brd4184a.overlay
@@ -4,11 +4,11 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/mr_canhubk3.overlay b/tests/drivers/i2c/i2c_target_api/boards/mr_canhubk3.overlay
index 9bf7ca16907..7fbae6b4907 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/mr_canhubk3.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/mr_canhubk3.overlay
@@ -11,7 +11,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -20,6 +20,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f091rc.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f091rc.overlay
index 0e2323a1a0f..9e6db75d1df 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f091rc.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f091rc.overlay
@@ -14,7 +14,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -25,7 +25,7 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f207zg.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f207zg.overlay
index fc88c69010b..2738f68845b 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f207zg.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f207zg.overlay
@@ -14,7 +14,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -22,6 +22,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f401re.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f401re.overlay
index 5c65f872319..8fdd8b2233d 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f401re.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f401re.overlay
@@ -14,7 +14,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -22,6 +22,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f429zi.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f429zi.overlay
index fc88c69010b..2738f68845b 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f429zi.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f429zi.overlay
@@ -14,7 +14,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -22,6 +22,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.overlay
index 8e740ab17af..4da288f9df6 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_f746zg.overlay
@@ -17,7 +17,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -25,6 +25,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_g071rb.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_g071rb.overlay
index b0d22a505fd..fb14393c879 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_g071rb.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_g071rb.overlay
@@ -17,7 +17,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -25,6 +25,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_g474re.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_g474re.overlay
index 4032d79c28e..2caffcbaa42 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_g474re.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_g474re.overlay
@@ -14,7 +14,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -25,6 +25,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_l073rz.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_l073rz.overlay
index db6f83236cb..9c067ae8b5c 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_l073rz.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_l073rz.overlay
@@ -14,7 +14,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -23,6 +23,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_l152re.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_l152re.overlay
index 2aa0dbcf233..aeabefb7e27 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_l152re.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_l152re.overlay
@@ -14,7 +14,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -26,6 +26,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_wb55rg.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_wb55rg.overlay
index 121265cad7c..bb73929888a 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_wb55rg.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_wb55rg.overlay
@@ -14,7 +14,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -26,6 +26,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/nucleo_wl55jc.overlay b/tests/drivers/i2c/i2c_target_api/boards/nucleo_wl55jc.overlay
index 333f50715b9..12445bcfe6f 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/nucleo_wl55jc.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/nucleo_wl55jc.overlay
@@ -14,7 +14,7 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -26,6 +26,6 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/rpi_pico.overlay b/tests/drivers/i2c/i2c_target_api/boards/rpi_pico.overlay
index ee5633c7088..f5e5f253898 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/rpi_pico.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/rpi_pico.overlay
@@ -18,7 +18,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -30,6 +30,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/stm32f072b_disco.overlay b/tests/drivers/i2c/i2c_target_api/boards/stm32f072b_disco.overlay
index 12ce645b511..bf249a3bb76 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/stm32f072b_disco.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/stm32f072b_disco.overlay
@@ -4,7 +4,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -13,6 +13,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/stm32f3_disco.overlay b/tests/drivers/i2c/i2c_target_api/boards/stm32f3_disco.overlay
index b23520b04ac..5c8f04c1022 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/stm32f3_disco.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/stm32f3_disco.overlay
@@ -16,7 +16,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -24,7 +24,7 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/stm32h573i_dk.overlay b/tests/drivers/i2c/i2c_target_api/boards/stm32h573i_dk.overlay
index 804f97c1a73..343edb771ad 100644
--- a/tests/drivers/i2c/i2c_target_api/boards/stm32h573i_dk.overlay
+++ b/tests/drivers/i2c/i2c_target_api/boards/stm32h573i_dk.overlay
@@ -17,7 +17,7 @@
 	eeprom0: eeprom@54 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x54>;
-		size = <1024>;
+		size = <256>;
 	};
 };
 
@@ -25,6 +25,6 @@
 	eeprom1: eeprom@56 {
 		compatible = "zephyr,i2c-target-eeprom";
 		reg = <0x56>;
-		size = <1024>;
+		size = <256>;
 	};
 };