diff --git a/tests/drivers/adc/adc_api/boards/b_u585i_iot02a_adc4.overlay b/tests/drivers/adc/adc_api/boards/b_u585i_iot02a_adc4.overlay
new file mode 100644
index 00000000000..3020a0c5997
--- /dev/null
+++ b/tests/drivers/adc/adc_api/boards/b_u585i_iot02a_adc4.overlay
@@ -0,0 +1,38 @@
+/*
+ * SPDX-License-Identifier: Apache-2.0
+ *
+ * Copyright (c) 2023 STMicroelectronics
+ */
+
+/ {
+	zephyr,user {
+		/* adjust channel number according to pinmux in board.dts */
+		io-channels = <&adc4 18>, <&adc4 19>;
+	};
+};
+
+&adc4 {
+	pinctrl-0 = <&adc4_in18_pb0 &adc4_in19_pb1>;
+	#address-cells = <1>;
+	#size-cells = <0>;
+
+	channel@12 {
+		reg = <18>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <ADC_ACQ_TIME_MAX>;
+		zephyr,resolution = <12>;
+	};
+
+	channel@13 {
+		reg = <19>;
+		zephyr,gain = "ADC_GAIN_1";
+		zephyr,reference = "ADC_REF_INTERNAL";
+		zephyr,acquisition-time = <ADC_ACQ_TIME_MAX>;
+		zephyr,resolution = <12>;
+	};
+};
+
+&adc1 {
+	status = "disabled";
+};
diff --git a/tests/drivers/adc/adc_api/testcase.yaml b/tests/drivers/adc/adc_api/testcase.yaml
index b29afdf561c..5e8217cb2c3 100644
--- a/tests/drivers/adc/adc_api/testcase.yaml
+++ b/tests/drivers/adc/adc_api/testcase.yaml
@@ -7,3 +7,8 @@ tests:
   drivers.adc:
     depends_on: adc
     min_flash: 40
+  drivers.adc.b_u585i_iot02a_adc4:
+    extra_args:
+      DTC_OVERLAY_FILE="boards/b_u585i_iot02a_adc4.overlay"
+    platform_allow:
+      - b_u585i_iot02a