diff --git a/doc/develop/test/twister.rst b/doc/develop/test/twister.rst
index 3be0132b05c..4773a3e3466 100644
--- a/doc/develop/test/twister.rst
+++ b/doc/develop/test/twister.rst
@@ -1082,6 +1082,10 @@ can be used multiple times and all given fixtures will be appended as a list. An
 given fixtures will be assigned to all boards, this means that all boards set by
 current twister command can run those testcases which request the same fixtures.
 
+Some fixtures allow for configuration strings to be appended, separated from the
+fixture name by a ``:``. Only the fixture name is matched against the fixtures
+requested by testcases.
+
 Notes
 +++++
 
diff --git a/scripts/pylib/twister/twisterlib/handlers.py b/scripts/pylib/twister/twisterlib/handlers.py
index 3a3c8de4cb3..4a9ed97ea2d 100755
--- a/scripts/pylib/twister/twisterlib/handlers.py
+++ b/scripts/pylib/twister/twisterlib/handlers.py
@@ -456,7 +456,7 @@ class DeviceHandler(Handler):
         dut_found = False
 
         for d in self.duts:
-            if fixture and fixture not in d.fixtures:
+            if fixture and fixture not in map(lambda f: f.split(sep=':')[0], d.fixtures):
                 continue
             if d.platform != device or (d.serial is None and d.serial_pty is None):
                 continue
diff --git a/scripts/pylib/twister/twisterlib/testinstance.py b/scripts/pylib/twister/twisterlib/testinstance.py
index fb0fc1d909b..b7a46fd5008 100644
--- a/scripts/pylib/twister/twisterlib/testinstance.py
+++ b/scripts/pylib/twister/twisterlib/testinstance.py
@@ -180,7 +180,7 @@ class TestInstance:
             # command-line, then we need to run the test, not just build it.
             fixture = testsuite.harness_config.get('fixture')
             if fixture:
-                can_run = fixture in fixtures
+                can_run = fixture in map(lambda f: f.split(sep=':')[0], fixtures)
 
         return can_run