diff --git a/samples/drivers/mbox/CMakeLists.txt b/samples/drivers/mbox/CMakeLists.txt
index ca6513822a8..ec79db9deed 100644
--- a/samples/drivers/mbox/CMakeLists.txt
+++ b/samples/drivers/mbox/CMakeLists.txt
@@ -17,7 +17,6 @@ if(("${BOARD}" STREQUAL "nrf5340dk_nrf5340_cpuapp") OR
    ("${BOARD}" STREQUAL "mimxrt1170_evkb_cm7") OR
    ("${BOARD}" STREQUAL "mimxrt1170_evk_cm7") OR
    ("${BOARD}" STREQUAL "mimxrt1160_evk_cm7") OR
-   ("${BOARD}" STREQUAL "mimxrt595_evk_cm33") OR
    ("${BOARD}" STREQUAL "lpcxpresso55s69_cpu0"))
   message(STATUS "${BOARD} compile as Main in this sample")
 else()
diff --git a/samples/drivers/mbox/Kconfig.sysbuild b/samples/drivers/mbox/Kconfig.sysbuild
index 20903872c34..a17dd56ee55 100644
--- a/samples/drivers/mbox/Kconfig.sysbuild
+++ b/samples/drivers/mbox/Kconfig.sysbuild
@@ -10,7 +10,6 @@ string
 	default "nrf5340dk_nrf5340_cpunet" if $(BOARD) = "nrf5340dk_nrf5340_cpuapp"
 	default "nrf5340bsim_nrf5340_cpunet" if $(BOARD) = "nrf5340bsim_nrf5340_cpuapp"
 	default "adp_xc7k_ae350" if $(BOARD) = "adp_xc7k_ae350"
-	default "nrf5340dk_nrf5340_cpunet" if $(BOARD) = "mimxrt595_evk_cm33"
 	default "mimxrt1170_evkb_cm4" if $(BOARD) = "mimxrt1170_evkb_cm7"
 	default "mimxrt1170_evk_cm4" if $(BOARD) = "mimxrt1170_evk_cm7"
 	default "mimxrt1160_evk_cm4" if $(BOARD) = "mimxrt1160_evk_cm7"
diff --git a/samples/drivers/mbox/boards/mimxrt595_evk_cm33.conf b/samples/drivers/mbox/boards/mimxrt595_evk_cm33.conf
deleted file mode 100644
index b01ff58e024..00000000000
--- a/samples/drivers/mbox/boards/mimxrt595_evk_cm33.conf
+++ /dev/null
@@ -1 +0,0 @@
-CONFIG_MBOX_NXP_IMX_MU=y
diff --git a/samples/drivers/mbox/sample.yaml b/samples/drivers/mbox/sample.yaml
index d646b241e9e..e38deda55d3 100644
--- a/samples/drivers/mbox/sample.yaml
+++ b/samples/drivers/mbox/sample.yaml
@@ -8,7 +8,6 @@ tests:
     platform_allow:
       - nrf5340dk_nrf5340_cpuapp
       - adp_xc7k_ae350
-      - mimxrt595_evk_cm33
       - mimxrt1170_evkb_cm7
       - mimxrt1170_evk_cm7
       - mimxrt1160_evk_cm7