diff --git a/samples/subsys/uuid/CMakeLists.txt b/samples/subsys/uuid/CMakeLists.txt
new file mode 100644
index 00000000000..98b06b94203
--- /dev/null
+++ b/samples/subsys/uuid/CMakeLists.txt
@@ -0,0 +1,9 @@
+# Copyright (c) 2025, SECO Mind Srl
+# SPDX-License-Identifier: Apache-2.0
+
+cmake_minimum_required(VERSION 3.20.0)
+find_package(Zephyr REQUIRED HINTS $ENV{ZEPHYR_BASE})
+project(dap)
+
+FILE(GLOB app_sources src/*.c)
+target_sources(app PRIVATE ${app_sources})
diff --git a/samples/subsys/uuid/README.rst b/samples/subsys/uuid/README.rst
new file mode 100644
index 00000000000..3e399af2d55
--- /dev/null
+++ b/samples/subsys/uuid/README.rst
@@ -0,0 +1,36 @@
+.. zephyr:code-sample:: uuid
+   :name: UUID
+
+   Manipulate UUID v4 and v5 compliant with IETF RFC 9562.
+
+Overview
+********
+
+This sample app demonstrates the use of the UUID utilities to generate and manipulate UUIDs
+accordingly to IETF RFC 9562.
+
+The following functionality is demonstrated:
+- UUIDv4 generation
+- UUIDv5 generation from namespace and data
+- UUID conversion from/to string and to base64 and base64 URL safe formats
+
+Requirements
+************
+
+This sample relies on the following modules:
+- MbedTLS for the UUIDv5 hash functions
+- Base64 for the base64 encoding of UUIDs
+- Entropy source for the pseudo-random generation of UUIDv4
+
+Building and Running
+********************
+
+Use the standard ``west`` commands to build and flash this application.
+For example for ``native_sim`` build with:
+```
+west build -p -b native_sim samples/subsys/uuid
+```
+Then run with:
+```
+west build -t run
+```
diff --git a/samples/subsys/uuid/prj.conf b/samples/subsys/uuid/prj.conf
new file mode 100644
index 00000000000..e68bba789fb
--- /dev/null
+++ b/samples/subsys/uuid/prj.conf
@@ -0,0 +1,13 @@
+CONFIG_UUID=y
+CONFIG_UUID_V5=y
+CONFIG_UUID_V4=y
+CONFIG_UUID_BASE64=y
+
+CONFIG_ENTROPY_GENERATOR=y
+
+CONFIG_MBEDTLS=y
+CONFIG_MBEDTLS_MD=y
+CONFIG_MBEDTLS_SHA1=y
+CONFIG_BASE64=y
+
+CONFIG_LOG=y
diff --git a/samples/subsys/uuid/sample.yaml b/samples/subsys/uuid/sample.yaml
new file mode 100644
index 00000000000..b8ec922c994
--- /dev/null
+++ b/samples/subsys/uuid/sample.yaml
@@ -0,0 +1,17 @@
+sample:
+  name: UUID
+tests:
+  sample.subsys.uuid:
+    tags: uuid
+    platform_allow:
+      - qemu_x86
+      - native_sim
+      - native_sim/native/64
+    integration_platforms:
+      - native_sim
+    filter: CONFIG_ENTROPY_HAS_DRIVER
+    harness: console
+    harness_config:
+      type: one_line
+      regex:
+        - "UUID sample completed successfully"
diff --git a/samples/subsys/uuid/src/main.c b/samples/subsys/uuid/src/main.c
new file mode 100644
index 00000000000..436e790e562
--- /dev/null
+++ b/samples/subsys/uuid/src/main.c
@@ -0,0 +1,75 @@
+/*
+ * Copyright (c) 2025, SECO Mind Srl
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+#include <zephyr/kernel.h>
+#include <zephyr/sys/uuid.h>
+
+#include <zephyr/logging/log.h>
+LOG_MODULE_REGISTER(uuid_sample, LOG_LEVEL_INF);
+
+int main(void)
+{
+	int result;
+	struct uuid uuid_v4 = {0};
+	struct uuid uuid_v5_namespace = {0};
+	struct uuid uuid_v5 = {0};
+	char uuid_v4_str[UUID_STR_LEN] = {0};
+	char uuid_v5_str[UUID_STR_LEN] = {0};
+	char uuid_v4_base64[UUID_BASE64_LEN] = {0};
+	char uuid_v4_base64url[UUID_BASE64URL_LEN] = {0};
+
+	/* Generate an UUID v4 from pseudo-random data */
+	result = uuid_generate_v4(&uuid_v4);
+	if (result != 0) {
+		LOG_ERR("UUID v4 generation failed, error: %s (%d)", strerror(result), result);
+		return -1;
+	}
+	/* Convert the UUID to string and to its base 64 and base 64 URL safe formats */
+	result = uuid_to_string(&uuid_v4, uuid_v4_str);
+	if (result != 0) {
+		LOG_ERR("UUID v4 to string failed, error: %s (%d)", strerror(result), result);
+		return -1;
+	}
+	LOG_INF("UUID v4: '%s'", uuid_v4_str);
+	result = uuid_to_base64(&uuid_v4, uuid_v4_base64);
+	if (result != 0) {
+		LOG_ERR("UUID v4 to base 64 failed, error: %s (%d)", strerror(result), result);
+		return -1;
+	}
+	LOG_INF("UUID v4 base 64: '%s'", uuid_v4_base64);
+	result = uuid_to_base64url(&uuid_v4, uuid_v4_base64url);
+	if (result != 0) {
+		LOG_ERR("UUID v4 to base 64 URL safe failed, error: %s (%d)", strerror(result),
+			result);
+		return -1;
+	}
+	LOG_INF("UUID v4 base 64 URL safe: '%s'", uuid_v4_base64url);
+
+	/* Generate an UUID v5 */
+	/* This UUID is the same as in RFC 9562 Appendix A.4: "Example of a UUIDv5 Value" */
+	result = uuid_from_string("6ba7b810-9dad-11d1-80b4-00c04fd430c8", &uuid_v5_namespace);
+	if (result != 0) {
+		LOG_ERR("Namespace string to UUID failed, error: %s (%d)", strerror(result),
+			result);
+		return -1;
+	}
+	result = uuid_generate_v5(&uuid_v5_namespace, "www.example.com", strlen("www.example.com"),
+				  &uuid_v5);
+	if (result != 0) {
+		LOG_ERR("UUID v5 generation failed, error: %s (%d)", strerror(result), result);
+		return -1;
+	}
+	result = uuid_to_string(&uuid_v5, uuid_v5_str);
+	if (result != 0) {
+		LOG_ERR("UUID v4 to string failed, error: %s (%d)", strerror(result), result);
+		return -1;
+	}
+	LOG_INF("UUID v5: '%s'", uuid_v5_str);
+
+	LOG_INF("UUID sample completed successfully");
+
+	return 0;
+}
diff --git a/tests/lib/uuid/src/main.c b/tests/lib/uuid/src/main.c
index 13fcaf6637b..b8607767e40 100644
--- a/tests/lib/uuid/src/main.c
+++ b/tests/lib/uuid/src/main.c
@@ -1,5 +1,5 @@
 /*
- * Copyright (c) 2024, SECO Mind Srl
+ * Copyright (c) 2025, SECO Mind Srl
  *
  * SPDX-License-Identifier: Apache-2.0
  */