diff --git a/boards/arm/b_u585i_iot02a/arduino_r3_connector.dtsi b/boards/arm/b_u585i_iot02a/arduino_r3_connector.dtsi
index 438187906d6..4bf599c616c 100644
--- a/boards/arm/b_u585i_iot02a/arduino_r3_connector.dtsi
+++ b/boards/arm/b_u585i_iot02a/arduino_r3_connector.dtsi
@@ -36,3 +36,4 @@
 };
 
 arduino_spi: &spi1 {};
+arduino_i2c: &i2c1 {};
diff --git a/boards/arm/b_u585i_iot02a/b_u585i_iot02a.dts b/boards/arm/b_u585i_iot02a/b_u585i_iot02a.dts
index 2ef68c6c36a..261182e122a 100644
--- a/boards/arm/b_u585i_iot02a/b_u585i_iot02a.dts
+++ b/boards/arm/b_u585i_iot02a/b_u585i_iot02a.dts
@@ -36,3 +36,21 @@
 		     &spi1_miso_pe14 &spi1_mosi_pe15>;
 	status = "okay";
 };
+
+&i2c1 {
+	pinctrl-0 = <&i2c1_scl_pb8 &i2c1_sda_pb9>;
+	status = "okay";
+	clock-frequency = <I2C_BITRATE_FAST>;
+};
+
+&i2c2 {
+	pinctrl-0 = <&i2c2_scl_ph4 &i2c2_sda_ph5>;
+	status = "okay";
+	clock-frequency = <I2C_BITRATE_FAST>;
+
+	hts221@5f {
+		compatible = "st,hts221";
+		reg = <0x5f>;
+		label = "HTS221";
+	};
+};
diff --git a/boards/arm/b_u585i_iot02a/b_u585i_iot02a.yaml b/boards/arm/b_u585i_iot02a/b_u585i_iot02a.yaml
index a6e4b2b3733..5158aacd737 100644
--- a/boards/arm/b_u585i_iot02a/b_u585i_iot02a.yaml
+++ b/boards/arm/b_u585i_iot02a/b_u585i_iot02a.yaml
@@ -9,5 +9,7 @@ toolchain:
 ram: 786
 flash: 2048
 supported:
+  - arduino_i2c
   - arduino_spi
+  - hts221
   - spi
diff --git a/boards/arm/b_u585i_iot02a/doc/index.rst b/boards/arm/b_u585i_iot02a/doc/index.rst
index 830e81baa22..de3450836c9 100644
--- a/boards/arm/b_u585i_iot02a/doc/index.rst
+++ b/boards/arm/b_u585i_iot02a/doc/index.rst
@@ -175,10 +175,11 @@ The Zephyr b_u585i_iot02a board configuration supports the following hardware fe
 +-----------+------------+-------------------------------------+
 | GPIO      | on-chip    | gpio                                |
 +-----------+------------+-------------------------------------+
+| I2C       | on-chip    | i2c                                 |
++-----------+------------+-------------------------------------+
 | SPI       | on-chip    | spi                                 |
 +-----------+------------+-------------------------------------+
 
-
 The default configuration can be found in the defconfig file:
 
 	``boards/arm/b_u585i_iot02a/b_u585i_iot02a_defconfig``
@@ -200,6 +201,8 @@ Default Zephyr Peripheral Mapping:
 - LD2 : PH6
 - user button : PC13
 - SPI1 NSS/SCK/MISO/MOSI : PE12/P13/P14/P15 (Arduino SPI)
+- I2C_1 SDA/SDL : PB9/PB8 (Arduino I2C)
+- I2C_2 SDA/SDL : PH5/PH4
 
 System Clock
 ------------