diff --git a/boards/st/nucleo_h745zi_q/doc/index.rst b/boards/st/nucleo_h745zi_q/doc/index.rst
index b1593b5e484..a77ac60e3f5 100644
--- a/boards/st/nucleo_h745zi_q/doc/index.rst
+++ b/boards/st/nucleo_h745zi_q/doc/index.rst
@@ -117,6 +117,8 @@ features:
 +-------------+------------+-------------------------------------+
 | USB OTG FS  | on-chip    | USB device                          |
 +-------------+------------+-------------------------------------+
+| SPI         | on-chip    | spi                                 |
++-------------+------------+-------------------------------------+
 
 Other hardware features are not yet supported on this Zephyr port.
 
@@ -138,6 +140,7 @@ and a ST morpho connector. Board is configured as follows:
 - LD2 : PB7
 - LD3 : PB14
 - I2C : PB8, PB9
+- SPI : PA5, PA6, PB5, PD14
 
 System Clock
 ------------
diff --git a/boards/st/nucleo_h745zi_q/nucleo_h745zi_q_stm32h745xx_m4.dts b/boards/st/nucleo_h745zi_q/nucleo_h745zi_q_stm32h745xx_m4.dts
index d4de60053ac..bca4302e2ff 100644
--- a/boards/st/nucleo_h745zi_q/nucleo_h745zi_q_stm32h745xx_m4.dts
+++ b/boards/st/nucleo_h745zi_q/nucleo_h745zi_q_stm32h745xx_m4.dts
@@ -35,3 +35,11 @@
 &rcc {
 	clock-frequency = <DT_FREQ_M(240)>;
 };
+
+&spi1 {
+	pinctrl-0 = <&spi1_sck_pa5 &spi1_miso_pa6 &spi1_mosi_pb5>;
+	pinctrl-names = "default";
+	cs-gpios = <&gpiod 14 (GPIO_ACTIVE_LOW | GPIO_PULL_UP)>;
+
+	status = "okay";
+};
diff --git a/boards/st/nucleo_h745zi_q/nucleo_h745zi_q_stm32h745xx_m7.dts b/boards/st/nucleo_h745zi_q/nucleo_h745zi_q_stm32h745xx_m7.dts
index d3ed100f74a..a09d693b029 100644
--- a/boards/st/nucleo_h745zi_q/nucleo_h745zi_q_stm32h745xx_m7.dts
+++ b/boards/st/nucleo_h745zi_q/nucleo_h745zi_q_stm32h745xx_m7.dts
@@ -132,6 +132,13 @@
 	status = "okay";
 };
 
+&spi1 {
+	pinctrl-0 = <&spi1_sck_pa5 &spi1_miso_pa6 &spi1_mosi_pb5>;
+	pinctrl-names = "default";
+	cs-gpios = <&gpiod 14 (GPIO_ACTIVE_LOW | GPIO_PULL_UP)>;
+	status = "okay";
+};
+
 zephyr_udc0: &usbotg_fs {
 	pinctrl-0 = <&usb_otg_fs_dm_pa11 &usb_otg_fs_dp_pa12>;
 	pinctrl-names = "default";
diff --git a/tests/drivers/spi/spi_loopback/testcase.yaml b/tests/drivers/spi/spi_loopback/testcase.yaml
index 5a00e2747c5..1cdb46beca6 100644
--- a/tests/drivers/spi/spi_loopback/testcase.yaml
+++ b/tests/drivers/spi/spi_loopback/testcase.yaml
@@ -56,6 +56,8 @@ tests:
     platform_allow:
       - nucleo_h743zi
       - nucleo_h753zi
+      - nucleo_h745zi_q/stm32h745xx/m4
+      - nucleo_h745zi_q/stm32h745xx/m7
   drivers.spi.stm32_spi_dma.loopback:
     extra_args: OVERLAY_CONFIG="overlay-stm32-spi-dma.conf"
     filter: CONFIG_SOC_FAMILY_STM32
@@ -71,6 +73,8 @@ tests:
       - nucleo_wl55jc
       - nucleo_h743zi
       - nucleo_h753zi
+      - nucleo_h745zi_q/stm32h745xx/m4
+      - nucleo_h745zi_q/stm32h745xx/m7
       - stm32h573i_dk
     integration_platforms:
       - nucleo_g474re
@@ -83,6 +87,8 @@ tests:
     platform_allow:
       - nucleo_h743zi
       - nucleo_h753zi
+      - nucleo_h745zi_q/stm32h745xx/m4
+      - nucleo_h745zi_q/stm32h745xx/m7
   drivers.spi.stm32_spi_16bits_frames_dma.loopback:
     extra_args:
       - OVERLAY_CONFIG="overlay-stm32-spi-16bits-dma.conf"
@@ -91,6 +97,8 @@ tests:
     platform_allow:
       - nucleo_h743zi
       - nucleo_h753zi
+      - nucleo_h745zi_q/stm32h745xx/m4
+      - nucleo_h745zi_q/stm32h745xx/m7
   drivers.spi.stm32_spi_16bits_frames_dma_dt_nocache_mem.loopback:
     extra_args:
       - OVERLAY_CONFIG="overlay-stm32-spi-16bits-dma-dt-nocache-mem.conf"
@@ -99,6 +107,8 @@ tests:
     platform_allow:
       - nucleo_h743zi
       - nucleo_h753zi
+      - nucleo_h745zi_q/stm32h745xx/m4
+      - nucleo_h745zi_q/stm32h745xx/m7
   drivers.spi.stm32_spi_interrupt.loopback:
     extra_args: OVERLAY_CONFIG="overlay-stm32-spi-interrupt.conf"
     filter: CONFIG_SOC_FAMILY_STM32
@@ -111,6 +121,8 @@ tests:
       - nucleo_g474re
       - nucleo_h743zi
       - nucleo_h753zi
+      - nucleo_h745zi_q/stm32h745xx/m4
+      - nucleo_h745zi_q/stm32h745xx/m7
       - nucleo_l152re
       - nucleo_wb55rg
       - nucleo_wl55jc