diff --git a/boards/arm/stm32l562e_dk/arduino_r3_connector.dtsi b/boards/arm/stm32l562e_dk/arduino_r3_connector.dtsi
index 87ee9535e22..0cbff1325ce 100644
--- a/boards/arm/stm32l562e_dk/arduino_r3_connector.dtsi
+++ b/boards/arm/stm32l562e_dk/arduino_r3_connector.dtsi
@@ -34,3 +34,5 @@
 			   <21 0 &gpiob 6 0>;	/* D15 */
 	};
 };
+
+arduino_spi: &spi3 {};
diff --git a/boards/arm/stm32l562e_dk/doc/index.rst b/boards/arm/stm32l562e_dk/doc/index.rst
index ab479289977..c83d1fd9a48 100644
--- a/boards/arm/stm32l562e_dk/doc/index.rst
+++ b/boards/arm/stm32l562e_dk/doc/index.rst
@@ -197,6 +197,7 @@ Default Zephyr Peripheral Mapping:
 - USART_1 TX/RX : PA9/PA10
 - I2C_1 SCL/SDA : PB6/PB7
 - SPI_1 SCK/MISO/MOSI : PG2/PG3/PG4 (BT SPI bus)
+- SPI_3 NSS/SCK/MISO/MOSI : PE0/PG9/PB4/PB5 (Arduino SPI)
 - USER_PB : PC13
 - LD10 : PG12
 - PWM_2_CH1 : PA0
diff --git a/boards/arm/stm32l562e_dk/stm32l562e_dk.yaml b/boards/arm/stm32l562e_dk/stm32l562e_dk.yaml
index ffc0ed8caf9..7c6c8b1b7d0 100644
--- a/boards/arm/stm32l562e_dk/stm32l562e_dk.yaml
+++ b/boards/arm/stm32l562e_dk/stm32l562e_dk.yaml
@@ -14,5 +14,7 @@ supported:
   - watchdog
   - dac
   - adc
+  - spi
+  - arduino_spi
 ram: 192
 flash: 512
diff --git a/boards/arm/stm32l562e_dk/stm32l562e_dk_common.dtsi b/boards/arm/stm32l562e_dk/stm32l562e_dk_common.dtsi
index 9577c2e0d90..e3f0e18e068 100644
--- a/boards/arm/stm32l562e_dk/stm32l562e_dk_common.dtsi
+++ b/boards/arm/stm32l562e_dk/stm32l562e_dk_common.dtsi
@@ -134,3 +134,9 @@
 	pinctrl-0 = <&adc1_in13_pc4>;
 	status = "okay";
 };
+
+&spi3 {
+	pinctrl-0 = <&spi3_sck_pg9 &spi3_mosi_pb5 &spi3_miso_pb4>;
+	cs-gpios = <&gpioe 0 (GPIO_ACTIVE_LOW | GPIO_PULL_UP)>;
+	status = "okay";
+};
diff --git a/boards/arm/stm32l562e_dk/stm32l562e_dk_ns.yaml b/boards/arm/stm32l562e_dk/stm32l562e_dk_ns.yaml
index 7af2ae999a9..44a97795807 100644
--- a/boards/arm/stm32l562e_dk/stm32l562e_dk_ns.yaml
+++ b/boards/arm/stm32l562e_dk/stm32l562e_dk_ns.yaml
@@ -11,5 +11,7 @@ supported:
   - lsm6dso
   - lptim
   - dac
+  - spi
+  - arduino_spi
 ram: 192
 flash: 512