diff --git a/tests/drivers/gpio/gpio_basic_api/boards/stm32l562e_dk.overlay b/tests/drivers/gpio/gpio_basic_api/boards/stm32l562e_dk.overlay
new file mode 100644
index 00000000000..37989a6bbb5
--- /dev/null
+++ b/tests/drivers/gpio/gpio_basic_api/boards/stm32l562e_dk.overlay
@@ -0,0 +1,13 @@
+/*
+ * Copyright (c) 2021 Linaro Limited
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/ {
+	resources {
+		compatible = "test,gpio_basic_api";
+		out-gpios = <&arduino_header 0 0>; /* Arduino A0 */
+		in-gpios = <&arduino_header 1 0>;  /* Arduino A1 */
+	};
+};