diff --git a/boards/arduino/uno_r4/arduino_uno_r4.dts b/boards/arduino/uno_r4/arduino_uno_r4.dts
index 09ce3664f1d..5551829a9b7 100644
--- a/boards/arduino/uno_r4/arduino_uno_r4.dts
+++ b/boards/arduino/uno_r4/arduino_uno_r4.dts
@@ -38,10 +38,19 @@
 	};
 };
 
+
+&ioport0 {
+	status = "okay";
+};
+
 &ioport1 {
 	status = "okay";
 };
 
+&ioport3 {
+	status = "okay";
+};
+
 &adc0 {
 	status = "okay";
 	pinctrl-0 = <&adc0_default>;
diff --git a/boards/arduino/uno_r4/arduino_uno_r4_wifi.overlay b/boards/arduino/uno_r4/arduino_uno_r4_wifi.overlay
index f411487d080..d102a016ced 100644
--- a/boards/arduino/uno_r4/arduino_uno_r4_wifi.overlay
+++ b/boards/arduino/uno_r4/arduino_uno_r4_wifi.overlay
@@ -94,6 +94,10 @@
 	};
 };
 
+&ioport4 {
+	status = "okay";
+};
+
 &spi0 {
 	pinctrl-0 = <&spi0_default>;
 	pinctrl-names = "default";