diff --git a/boards/adi/max32675evkit/max32675evkit.dts b/boards/adi/max32675evkit/max32675evkit.dts
index 1d7143aaf05..3c5bfccd8b5 100644
--- a/boards/adi/max32675evkit/max32675evkit.dts
+++ b/boards/adi/max32675evkit/max32675evkit.dts
@@ -85,6 +85,12 @@
 	status = "okay";
 };
 
+&i2c2 {
+	status = "okay";
+	pinctrl-0 = <&i2c2a_scl_p0_18 &i2c2a_sda_p0_19>;
+	pinctrl-names = "default";
+};
+
 &trng {
 	status = "okay";
 };
diff --git a/tests/drivers/i2c/i2c_target_api/boards/max32675evkit.conf b/tests/drivers/i2c/i2c_target_api/boards/max32675evkit.conf
new file mode 100644
index 00000000000..7fc1aaebd9c
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/max32675evkit.conf
@@ -0,0 +1,6 @@
+#
+# Copyright (c) 2024 Analog Devices, Inc.
+#
+# SPDX-License-Identifier: Apache-2.0
+#
+CONFIG_I2C_VIRTUAL=n
diff --git a/tests/drivers/i2c/i2c_target_api/boards/max32675evkit.overlay b/tests/drivers/i2c/i2c_target_api/boards/max32675evkit.overlay
new file mode 100644
index 00000000000..59b1258c557
--- /dev/null
+++ b/tests/drivers/i2c/i2c_target_api/boards/max32675evkit.overlay
@@ -0,0 +1,30 @@
+/*
+ * Copyright (c) 2024 Analog Devices, Inc.
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+&i2c0 {
+	status = "okay";
+	pinctrl-0 = <&i2c0a_scl_p0_6 &i2c0a_sda_p0_7>;
+	pinctrl-names = "default";
+
+	eeprom0: eeprom@54 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x54>;
+		address-width = <16>;
+		size = <1024>;
+	};
+};
+
+&i2c2 {
+	dmas = <&dma0 1 MAX32_DMA_SLOT_I2C2_TX>, <&dma0 2 MAX32_DMA_SLOT_I2C2_RX>;
+	dma-names = "tx", "rx";
+
+	eeprom1: eeprom@56 {
+		compatible = "zephyr,i2c-target-eeprom";
+		reg = <0x56>;
+		address-width = <16>;
+		size = <1024>;
+	};
+};